APPLICATIONS

One key to ensuring the best results are obtained with the ATF™ System is to appreciate the differences involved with each application where it is used. Understanding what is important for each application and where the improvements in efficiency lie may mean a shift in focus for operating the ATF System.

Insect cells and CHO cells have much common when grown in a bioreactor but also much separates them in how the ATF System can improve each individual process. Obtaining a high cell concentration may not be the goal of a vaccine process which cannot realize the benefit of more cells – but perhaps boosting virus numbers per cell is where the focus and effort for process improvement has the most impact.

Transient expression is a favored method of producing small quantities of protein fast. A concentrated fed-batch approach may provide an excitingly high cell concentration but this may not translate to a similar protein production spike. Shortening the time however to produce an equivalent amount of protein could be significant while improving the turnaround time and total output of the research group. A high density cell bank made using the ATF System provides this. The ATF System can double as a clarification device avoiding centrifugation and allowing a cell-free harvest straight from the Wave bag, Spinner or another simple reactor type.

See RELATED LINKS for examples of applications, the key benefits achievable with the ATF System, plus some case studies.