ADHERENT CELLS

Microcarriers
Wash, Concentration, Perfusion, Rapid Medium Exchange, Trypsinisation, Wash, Cell-Microcarrier Separation, Virus Perfusion, Virus Harvest.

Microcarrier processes are inherently more complex and more involved than concentrated fed-batch or fed-batch processes. The extra steps required for preparation of the vessel and various washing procedures during the process often means that there is significant manual intervention and multiple points of potential contamination.

The ATF™ System simplifies and makes reliable almost every complex step in an adherent cell-line process which uses microcarriers such as Cytodex. From washing the carriers post-sterilisation, to perfusion, to cell-microcarrier separations and the final harvest, the ATF System offers significant benefits. The benefits arise due to a specially designed single-use 70µ screen module filter which is inserted into the ATF System housing allowing it to operate as an efficient macro-filter. The same filter is used throughout the process, while the ATF System is fully automatic for all process steps at almost all flows. The ATF System for microcarrier processes scales from 1L to 5000L and is also compatible with all single-use bioreactor types on the market today.